《植物生理学报》 2014, 50(10): 1510-1514

流苏树的组织培养和快速繁殖

王鑫, 孔祥生*

河南科技大学农学院, 河南洛阳471003

通信作者：孔祥生；E-mail: kxsh55@163.com；Tel: 13333870210

摘　要：

以流苏树种胚为试材, 研究不同种类及浓度的植物生长调节剂对其无菌体系建立, 带顶、侧芽茎段伸长以及生根的影响。结果表明, 种胚生根最适培养基为WPM+0.1 mg∙L^-1 NAA+3.0 mg∙L^-1 GA₃+2.0 mg∙L^-1 6-BA, 生根率为94.2%; 带顶、侧芽茎段最适培养基为WPM+1.0 mg∙L^-1 GA₃+1.0 mg∙L^-1 6-BA+0.5 mg∙L^-1 TDZ; 生根最适培养基为WPM+0.1 mg∙L^-1 6-BA+0.5 mg∙L^-1 NAA+0.5 mg∙L^-1 IBA。

关键词：流苏树; 种胚; 无菌体系

收稿：2014-04-11   修定：2014-09-17

资助：河南省科技攻关计划项目(090080021023)。

Tissue Culture and Rapid Propagation of Chionanthus retusus

WANG Xin, KONG Xiang-Sheng*

College of Agronomy, Henan University of Science and Technology, Luoyang, Henan 471003, China

Corresponding author: KONG Xiang-Sheng; E-mail: kxsh55@163.com; Tel: 13333870210

Abstract:

Using the embryo of Chionanthus retusus as the material, the effects of different concentrations and combinations of plant growth regulators on asepsis system establishment, stem segment elongation of terminal bud and lateral bud, and rooting were discussed. The results showed that the best embryo rooting medium was WPM+1.0 mg∙L^-1 NAA+3.0 mg∙L^-1 GA₃+2.0 mg∙L^-1 6-BA, the rooting rate was 94.2%, the best medium for terminal and laterd bud was WPM+1.0 mg∙L^-1 GA₃+1.0 mg∙L^-1 6-BA+0.5 mg∙L^-1 TDZ, and the best rooting medium was WPM+0.1 mg∙L^-1 6-BA+0.5 mg∙L^-1 NAA+0.5 mg∙L^-1 IBA.

Key words: Chionanthus retusus; embryo; tissue culture